Lane 1: Wild-type MDA-MB-231 whole cell lysate.
Lane 2: PKM knockout MDA-MB-231 whole cell lysate.
ER1901-90 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1901-90, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Applications
-
WB
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
PKM Rabbit Polyclonal Antibody (ER1901-90)
Immunogen
Synthetic peptide within human pkm aa 380-450.
Host
Rabbit
Positive Control
SiHa cell lysate, A549 cell lysate, PC-3M cell lysate, F9, MDA-MB-231, human kidney tissue, human placenta tissue, human womb tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
58 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
PKM
SYNONYMS
CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody
SEQUENCE SIMILARITIES
Belongs to the pyruvate kinase family.
TISSUE SPECIFICITY
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
POST-TRANSLATIONAL MODIFICATION
ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
SUBCELLULAR LOCATION
Nucleus, cytoplasm.
FUNCTION
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.