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PRODUCT CODE: ER1901-90

PKM Rabbit Polyclonal Antibody (ER1901-90)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

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All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1901-90) at 1:500 dilution.<br /> Lane 1: Wild-type MDA-MB-231 whole cell lysate.<br /> Lane 2: PKM knockout MDA-MB-231 whole cell lysate.<br /> <br /> ER1901-90 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1901-90, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1901-90) at 1:500 dilution.<br /> Lane 1: Wild-type MDA-MB-231 whole cell lysate.<br /> Lane 2: PKM knockout MDA-MB-231 whole cell lysate.<br /> <br /> ER1901-90 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1901-90, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PKM2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: SiHa cell lysate<br /> Lane 2: A549 cell lysate<br /> Lane 3: PC-3M cell lysate
  • Flow cytometric analysis of PKM2 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1901-90) at 1:500 dilution.
Lane 1: Wild-type MDA-MB-231 whole cell lysate.
Lane 2: PKM knockout MDA-MB-231 whole cell lysate.

ER1901-90 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1901-90, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PKM Rabbit Polyclonal Antibody (ER1901-90)

Immunogen

Synthetic peptide within human pkm aa 380-450.

Host

Rabbit

Positive Control

SiHa cell lysate, A549 cell lysate, PC-3M cell lysate, F9, MDA-MB-231, human kidney tissue, human placenta tissue, human womb tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • FC:1:50-1:100

TARGET

UNIPROT #

P14618

PROTEIN NAME

PKM

SYNONYMS

CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody

SEQUENCE SIMILARITIES

Belongs to the pyruvate kinase family.

TISSUE SPECIFICITY

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

SUBCELLULAR LOCATION

Nucleus, cytoplasm.

FUNCTION

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.