PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17] (ET1705-4)
Catalog# ET1705-4
Rabbit Monoclonal to PKC mu
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WB
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Human
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Mouse
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Rat
Western blot analysis of PKC mu on different lysates with Rabbit anti-PKC mu antibody (ET1705-4) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si PKC mu cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17] (ET1705-4)
Immunogen
Synthetic peptide within human pkc aa 201-250 / 912.
Host
Rabbit
Positive Control
Hela cell lysate, mouse kidney tissue lysate, PC-12 cell lysate, PC-3 cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM30-17
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PKC mu
SYNONYMS
KPCD1_HUMAN antibody;nPKC D1 antibody;nPKC mu antibody;nPKC-D1 antibody;nPKC-mu antibody;nPKCD1 antibody;nPKCmu antibody;PKC antibody;PKC MU antibody;PKCM antibody;PKCmu antibody;PKD 1 antibody;PKD antibody;PKD1 antibody;PRKCM antibody;PRKD 1 antibody;Prkd1 antibody;Protein kinase C mu antibody;Protein kinase C mu type antibody;Protein kinase D antibody;Protein kinase D1 antibody;Serine/threonine protein kinase D1 antibody;Serine/threonine-protein kinase D1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at Ser-397 and Ser-401 by MAPK13 during regulation of insulin secretion in pancreatic beta cells. Phosphorylated by DAPK1. Phosphorylated at Tyr-95 and by ABL at Tyr-463, which primes the kinase in response to oxidative stress, and promotes a second step activating phosphorylation at Ser-738/Ser-742 by PKRD. Phosphorylated on Ser-910 upon S.enterica infection in macrophages (By similarity).
SUBCELLULAR LOCATION
Cell membrane, trans-Golgi network, Cytoplasm.
FUNCTION
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response.
CITATIONS
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Chen Bo,et al
Cepharanthine inhibits migration, invasion, and EMT of bladder cancer cells by activating the Rap1 signaling pathway in vitro
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WB
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Human
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Citation
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