PKC gamma Recombinant Rabbit Monoclonal Antibody [JG69-32] (ET7108-42)
Catalog# ET7108-42
Rabbit Monoclonal to PKC gamma
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PKC gamma Recombinant Rabbit Monoclonal Antibody [JG69-32] (ET7108-42)
Immunogen
Recombinant protein within human pkc gamma aa 564-697 / 697.
Host
Rabbit
Positive Control
Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, A549 cell lysates, mouse cerebellum tissue, rat cerebellum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG69-32
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:1,000-1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 78 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PKC gamma
SYNONYMS
KPCG_HUMAN antibody;MGC57564 antibody;OTTHUMP00000067291 antibody;PKC-gamma antibody;PKCC antibody;PKCG antibody;PRKCG antibody;Protein kinase C gamma antibody;Protein kinase C gamma polypeptide antibody;Protein kinase C gamma type antibody;Protein kinase C, gamma antibody;SCA 14 antibody;SCA14 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
TISSUE SPECIFICITY
Expressed in Purkinje cells of the cerebellar cortex.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4.; Ubiquitinated.
SUBCELLULAR LOCATION
Cell membrane. Cytoplasm.
FUNCTION
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, µ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
CITATIONS
-
Zeng Qi,et al
Label-free Raman imaging for screening of anti-inflammatory function food
-
WB
-
Mouse
-
Citation
-
