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Western blot analysis of PKC alpha on different lysates with Rabbit anti-PKC alpha antibody (ET1608-15) at 1/500 dilution.
Lane 1: 293 cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Protein kinase C alpha (PKCα) is an enzyme that in humans is encoded by the PRKCA gene. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. Protein kinase C-alpha (PKC-α) is a specific member of the protein kinase family. These enzymes are characterized by their ability to add a phosphate group to other proteins, thus changing their function.
Liu, Suzi et al.
Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-κB pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie