PRODUCT CODE: ET7111-16

Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39] (ET7111-16)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

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Western blot analysis of Pirh2 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-16, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Pirh2 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-16, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Pirh2 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-16, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39] (ET7111-16)

Immunogen

Synthetic peptide within n-terminal human pirh2.

Host

Rabbit

Positive Control

293T cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE55-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

30 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Pirh2

SYNONYMS

Androgen receptor N terminal interacting protein antibody; Androgen receptor N-terminal-interacting protein antibody; ARNIP antibody; CH-rich-interacting match with PLAG1 antibody; CHIMP antibody; E3 ubiquitin-protein ligase Pirh2 antibody; hARNIP antibody; hPirh2 antibody; p53 induced protein with a RING H2 domain antibody; p53-induced RING-H2 protein antibody; PIRH2E antibody; PIRH2F antibody; PRO1996 antibody; RCHY1 antibody; Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase antibody; RING finger and CHY zinc finger domain-containing protein 1 antibody; RING finger protein 199 antibody; RNF199 antibody; ZCHY antibody; ZFP 363 antibody; zinc finger CHY type antibody; Zinc finger protein 363 antibody; ZN363_HUMAN antibody; ZNF363 antibody

SUBCELLULAR LOCATION

Nucleus, Nucleus speckle, Cytoplasm.

FUNCTION

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.