PRODUCT CODE: ET7108-60

PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80] (ET7108-60)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SK-Br-3 cell lysate<br />
Lane 2: 293 cell lysate
  • Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SK-Br-3 cell lysate<br />
Lane 2: 293 cell lysate
  • Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80] (ET7108-60)

Immunogen

Synthetic peptide within human pick1 aa 1-50 / 415.

Host

Rabbit

Positive Control

SK-Br-3 cell lysate, 293 cell lysate, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG30-80

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

PICK1

SYNONYMS

dJ1039K5 antibody; MGC15204 antibody; OTTHUMP00000028509 antibody; PICK 1 antibody; Pick1 antibody; PICK1_HUMAN antibody; PRKCA binding protein antibody; PRKCA-binding protein antibody; PRKCABP antibody; Protein interacting with C kinase 1 antibody; Protein interacting with PRKCA antibody; Protein interacting with PRKCA 1 antibody; Protein kinase C alpha binding protein antibody; Protein kinase C-alpha-binding protein antibody; Protein that interacts with C kinase 1 antibody

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.; Palmitoylation on Cys-413 is essential for long-term synaptic depression (LTD).

SUBCELLULAR LOCATION

cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome.

FUNCTION

Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCα activation. PKCα, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCα. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.