PIAS1 Recombinant Rabbit Monoclonal Antibody [JE30-36] (HA721035)

UNIPROT #

O75925

PROTEIN NAME

PIAS1

SYNONYMS

AR interacting protein antibody;DDXBP1 antibody;DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody;DEAD/H box binding protein 1 antibody;DEAD/H box-binding protein 1 antibody;E3 SUMO-protein ligase PIAS1 antibody;GBP antibody;Gu binding protein antibody;Gu-binding protein antibody;GU/RH-II antibody;Pias1 antibody;PIAS1_HUMAN antibody;Protein inhibitor of activated STAT protein 1 antibody;Protein inhibitor of activated STAT, 1 antibody;RNA helicase II binding protein antibody;RNA helicase II-binding protein antibody;Zinc finger, MIZ-type containing 3 antibody;ZMIZ3 antibody

SUBCELLULAR LOCATION

Nucleus, Nucleus speckle, Cytoplasm; Nucleus speckle, PML body, Nucleus; Nucleus speckle, PML body.

FUNCTION

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.