PRODUCT CODE: ET1704-20

Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49] (ET1704-20)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Phosphotyrosine on A431 cells lysates treated (<br />
  Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.
  • Western blot analysis of Phosphotyrosine on A431 cells lysates treated (<br />
  Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.
  • ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of A431 cells with Phosphotyrosine antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Phosphotyrosine on A431 cells lysates treated (
Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49] (ET1704-20)

Immunogen

Synthetic peptide conjugated to keyhole limpet haemocyanin (klh)

Host

Rabbit

Positive Control

A431 cells lysates treated with Sodium metavanadate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-49

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:50-1:200

  • FC

  • 1:10-1:50 ICC/IF

TARGET

PROTEIN NAME

Phosphotyrosine

FUNCTION

Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.