Phospho-YAP1 (S127) Recombinant Rabbit Monoclonal Antibody [SN0718] (ET1611-69)
Catalog# ET1611-69
Rabbit Monoclonal to Phospho-YAP1 (S127)
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Western blot analysis of Phospho-YAP1 (S127) on different lysates with Rabbit anti-Phospho-YAP1 (S127) antibody (ET1611-69) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: C6 cell lysate
Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 starved for 24 hours then treated with 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 70 kDa
Exposure time: 50 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-69) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-YAP1 (S127) Recombinant Rabbit Monoclonal Antibody [SN0718] (ET1611-69)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser127 of human yap1.
Host
Rabbit
Modification
Phospho
Modification Site
S127
Positive Control
HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, SiHa cell lysates, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN0718
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:2,000-1:5,000
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IF-Tissue
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1:50-1:400
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-YAP1 (S127)
SYNONYMS
65 kDa Yes associated protein antibody;65 kDa Yes-associated protein antibody;COB1 antibody;YAp 1 antibody;YAP 65 antibody;YAP antibody;YAP1 antibody;YAP1_HUMAN antibody;YAP2 antibody;YAP65 antibody;yes -associated protein delta antibody;Yes associated protein 1 65kDa antibody;Yes associated protein 1 antibody;Yes associated protein 2 antibody;yes associated protein beta antibody;YKI antibody;Yorkie homolog antibody
SEQUENCE SIMILARITIES
Belongs to the YAP1 family.
TISSUE SPECIFICITY
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1.; Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.
CITATIONS
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Zhang, G., Dai, S., Chen, Y., Wang, H., Chen, T., Shu, Q., Chen, S., Shou, L., & Cai, X.
Aqueous extract of Taxus chinensis var. mairei regulates the Hippo-YAP pathway and promotes apoptosis of non-small cell lung cancer via ATF3 in vivo and in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111506.
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WB
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Mouse
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Human
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Citation
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ANNEXIN A2 FACILITATES NEOVASCULARIZATION TO PROTECT AGAINST MYOCARDIAL INFARCTION INJURY VIA INTERACTING WITH MACROPHAGE YAP AND ENDOTHELIAL INTEGRIN Β3
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WB
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Mouse
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Citation
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Xinyi Wang,et al
Macrophage-derived amphiregulin promoted the osteogenic differentiation of chondrocytes through EGFR/Yap axis and TGF-β activation
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WB
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Citation
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Xie Binqing,et al
Cyclic tensile stress promotes osteogenic differentiation via upregulation of Piezo1 in human dental follicle stem cells
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WB
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Human
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Citation
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Yao, W., Ma, A., Zhang, Z., & Zhu, L.
Intermittent Hydrostatic Pressure Promotes Cartilage Repair in an Inflammatory Environment through Hippo-YAP Signaling In Vitro and In Vivo
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WB
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IHC-P
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Rat
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Citation
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