Phospho-SYK (Y525/526) Recombinant Rabbit Monoclonal Antibody [PSH03-70] (HA722034)
Catalog# HA722034
Rabbit Monoclonal to Phospho-SYK (Y525/526)
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-SYK (Y525 + Y526) on different lysates with Rabbit anti-Phospho-SYK (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: Lane 1-2 (left): 1 minute 2 seconds; Lane 1-2 (right): 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Ramos cell lysate
Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: Lane 1-2 (left): 1 minute 2 seconds; Lane 1-2 (right): 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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