Phospho-Smad5 (S463 + S465) Recombinant Rabbit Monoclonal Antibody [SY09-03] (ET1605-5)
Catalog# ET1605-5
Rabbit Monoclonal to Phospho-Smad5 (S463/465)
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
Western blot analysis of Phospho-Smad5 (S463 + S465) on different lysates with Rabbit anti-Phospho-Smad5 (S463 + S465) antibody (ET1605-5) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Smad5 (S463 + S465) Recombinant Rabbit Monoclonal Antibody [SY09-03] (ET1605-5)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser463 and 465 of human smad5. the immunogen may cross with p-smad1/9.
Specificity
Phospho-smad/5 (ser463/465) (sy09-03) rabbit mab detects endogenous levels of smad5 only when dually phosphorylated at ser463 and ser465 and is also predicted to detect smad1 when phosphorylated at ser463 and ser465. the antibody may be also predicted to detect smad9 (smad8) when phosphorylated at ser465 and ser467. the antibody does not cross-react with other smad-related proteins.
Host
Rabbit
Modification
Phospho
Modification Site
S463/465
Positive Control
HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate, Rat brain tissue lysate, mouse brain tissue lysate, HeLa cells treated with 20ng/mL TGFβ1 for 15 minutes, SKOV-3, HepG2, human tonsil tissue, human breast carcinoma tissue, human liver tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY09-03
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 52 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Smad5 (S463/465)
SYNONYMS
DKFZp781C1895 antibody;DKFZp781O1323 antibody;Dwfc antibody;hSmad5 antibody;JV5 1 antibody;JV5-1 antibody;MAD homolog 5 antibody;MAD, mothers against decapentaplegic homolog 5 antibody;MADH 5 antibody;MADH5 antibody;Mothers against decapentaplegic homolog 5 antibody;mothers against decapentaplegic, drosophila, homolog of, 5 antibody;Mothers against DPP homolog 5 antibody;MusMLP antibody;SMA and MAD related protein 5 antibody;SMAD 5 antibody;SMAD family member 5 antibody;SMAD, mothers against DPP homolog 5 antibody;Smad5 antibody;SMAD5_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the dwarfin/SMAD family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.; Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
CITATIONS
-
Huang, X., Pan, X., Zhang, B., Huang, W., Cen, X., Liu, J., & Zhao, Z.
CircRFWD2 Promotes Osteogenic Differentiation of human Dental Pulp Stem Cells by Targeting miR-6817-5p Through BMP-Smad and p38 MAPK Pathway
-
WB
-
Human
-
Citation
-
-
Cen, X., Pan, X., Zhang, B., Huang, W., Pei, F., Luo, T., Huang, X., Liu, J., & Zhao, Z.
miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38
-
WB
-
Human
-
Citation
-
