PRODUCT CODE: ET1701-5

Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03] (ET1701-5)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

ICC staining Phospho-SHIP(Y1020) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-SHIP(Y1020) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-SHIP(Y1020) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-SHIP(Y1020) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Phospho-SHIP(Y1020) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03] (ET1701-5)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding tyr1020 of human ship.

Host

Rabbit

Modification

Phospho

Modification Site

Y1020

Positive Control

A549, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ082-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

133 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-SHIP (Y1020)

SYNONYMS

Inositol polyphosphate 5 phosphatase of 145kDa antibody; 4 antibody; 5-trisphosphate 5-phosphatase 1 antibody; hp51CN antibody; Inositol polyphosphate 5 phosphatase 145kDa antibody; Inositol polyphosphate 5 phosphatase antibody; Inositol polyphosphate-5-phosphatase of 145 kDa antibody; INPP5D antibody; MGC104855 antibody; MGC142140 antibody; MGC142142 antibody; p150Ship antibody; Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody; Phosphatidylinositol-3 antibody; SH2 containing inositol phosphatase isoform b antibody; SH2 domain containing inositol 5' phosphatase 1 antibody; SH2 domain containing inositol phosphatase 1 antibody; SH2 domain-containing inositol phosphatase 1 antibody; SH2 domain-containing inositol-5'-phosphatase 1 antibody; SHIP-1 antibody; SHIP1 antibody; SHIP1_HUMAN antibody; Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody; SIP-145 antibody; SIP145 antibody

SEQUENCE SIMILARITIES

Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.

TISSUE SPECIFICITY

Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

SUBCELLULAR LOCATION

Cytoplasm, Cell membrane, Membrane raft, Membrane.

FUNCTION

The major translational product of the v-Fms oncogene, originally isolated from the McDonough strain of feline sarcoma virus, has been identified as a glycoprotein with intrinsic tyrosine kinase activity. The v-Fms human cellular homolog, c-Fms, has been molecularly cloned and mapped to band q34 on chromosome 5, and identified as the receptor for hematopoietic ligand, CSF-1. Ligand-induced activation of the intrinsic CSF-1R protein tyrosine kinase triggers its interaction with cytoplasmic effector molecules. One such effector molecule, SHIP-1 p145 (SH2-containing-inositol phosphatase), associates with activated Fms. SHIP-1 contains two phosphotyrosine-binding domains (PTB), a unique amino terminal SH2 domain, a proline-rich region, and two highly conserved motifs found among inositol phosphate 5-phosphatases. SHIP-1 displays both phosphatidylinositol 3,4,5-triphosphate and inositol 1,3,4,5-tetrakisphosphate polyphosphate 5-phosphatase activity. Evidence suggests that SHIP-1 may modulate Ras signaling in addition to inositol signaling pathways.