Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)
Catalog# ET1610-28
Rabbit Monoclonal to Phospho-RSK1 (S380)
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WB
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IF-Cell
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IF-Tissue
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IP
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Human
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Mouse
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Rat
Western blot analysis of Phospho-RSK1 (S380) on different lysates with Rabbit anti-Phospho-RSK1 (S380) antibody (ET1610-28) at 1/500 dilution.
Lane 1: Hela whole cell lysate
Lane 2: Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-28) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding s380 of human rsk1.
Host
Rabbit
Modification
Phospho
Modification Site
S380
Positive Control
A431 cell lysates, Hela, A431, 293, Hela whole cell lysate, Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate, NIH/3T3 whole cell lysate, NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC05-32
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 83 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-RSK1 (S380)
SYNONYMS
90 kDa ribosomal protein S6 kinase 1 antibody;dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody;dJ590P13.1 antibody;EC 2.7.11.1 antibody;HU 1 antibody;HU1 antibody;KS6A1_HUMAN antibody;MAP kinase activated protein kinase 1a antibody;MAP kinase-activated protein kinase 1a antibody;MAPK-activated protein kinase 1a antibody;MAPKAP kinase 1a antibody;MAPKAPK-1a antibody;MAPKAPK1A antibody;MGC79981 antibody;Mitogen-activated protein kinase-activated protein kinase 1A antibody;OTTHUMP00000004113 antibody;p90 RSK1 antibody;p90-RSK 1 antibody;p90rsk antibody;p90RSK1 antibody;p90S6K antibody;pp90RSK1 antibody;Ribosomal protein S6 kinase 90kD 1 antibody;Ribosomal protein S6 kinase 90kD polypeptide 1 antibody;Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody;Ribosomal protein S6 kinase alpha 1 antibody;Ribosomal protein S6 kinase alpha-1 antibody;Ribosomal protein S6 kinase polypeptide 1 antibody;Ribosomal S6 kinase 1 antibody;RPS6K1 alpha antibody;rps6ka antibody;Rps6ka1 antibody;RSK 1 antibody;RSK 1 p90 antibody;RSK antibody;RSK-1 antibody;RSK1 antibody;S6K alpha 1 antibody;S6K-alpha-1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.
CITATIONS
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Zhou Nana,et al
PPP1R14B-mediated phosphorylation enhances protein stability of RPS6KA1 to promote hepatocellular carcinoma tumorigenesis
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WB
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Human
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Citation
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