PRODUCT CODE: ET1610-28

Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)

  • Recombinant

Applications

  • WB

  • ICC/IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
<br />
Predicted band size: 83 kDa<br />
Observed band size: 80 kDa
  • Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
<br />
Predicted band size: 83 kDa<br />
Observed band size: 80 kDa
  • ICC staining of Phospho-RSK1(S380) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Phospho-RSK1(S380) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Phospho-RSK1(S380) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 83 kDa
Observed band size: 80 kDa

Applications

  • WB

  • ICC/IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding s380 of human rsk1.

Host

Rabbit

Modification

Phospho

Modification Site

S380

Positive Control

A431 cell lysates, Hela, A431, 293.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC05-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 83 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-RSK1 (S380)

SYNONYMS

90 kDa ribosomal protein S6 kinase 1 antibody;dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody;dJ590P13.1 antibody;EC 2.7.11.1 antibody;HU 1 antibody;HU1 antibody;KS6A1_HUMAN antibody;MAP kinase activated protein kinase 1a antibody;MAP kinase-activated protein kinase 1a antibody;MAPK-activated protein kinase 1a antibody;MAPKAP kinase 1a antibody;MAPKAPK-1a antibody;MAPKAPK1A antibody;MGC79981 antibody;Mitogen-activated protein kinase-activated protein kinase 1A antibody;OTTHUMP00000004113 antibody;p90 RSK1 antibody;p90-RSK 1 antibody;p90rsk antibody;p90RSK1 antibody;p90S6K antibody;pp90RSK1 antibody;Ribosomal protein S6 kinase 90kD 1 antibody;Ribosomal protein S6 kinase 90kD polypeptide 1 antibody;Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody;Ribosomal protein S6 kinase alpha 1 antibody;Ribosomal protein S6 kinase alpha-1 antibody;Ribosomal protein S6 kinase polypeptide 1 antibody;Ribosomal S6 kinase 1 antibody;RPS6K1 alpha antibody;rps6ka antibody;Rps6ka1 antibody;RSK 1 antibody;RSK 1 p90 antibody;RSK antibody;RSK-1 antibody;RSK1 antibody;S6K alpha 1 antibody;S6K-alpha-1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.