PRODUCT CODE: ET1610-28

Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates using anti-Phospho-RSK1(S380) antibody at 1/1,000 dilution.
  • Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates using anti-Phospho-RSK1(S380) antibody at 1/1,000 dilution.
  • ICC staining Phospho-RSK1(S380) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-RSK1(S380) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-RSK1(S380) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Phospho-RSK1(S380) on A431 cell lysates using anti-Phospho-RSK1(S380) antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32] (ET1610-28)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Phospho

Modification Site

S380

Positive Control

A431, 293, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC05-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-RSK1 (S380)

SYNONYMS

90 kDa ribosomal protein S6 kinase 1 antibody; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody; dJ590P13.1 antibody; EC 2.7.11.1 antibody; HU 1 antibody; HU1 antibody; KS6A1_HUMAN antibody; MAP kinase activated protein kinase 1a antibody; MAP kinase-activated protein kinase 1a antibody; MAPK-activated protein kinase 1a antibody; MAPKAP kinase 1a antibody; MAPKAPK-1a antibody; MAPKAPK1A antibody; MGC79981 antibody; Mitogen-activated protein kinase-activated protein kinase 1A antibody; OTTHUMP00000004113 antibody; p90 RSK1 antibody; p90-RSK 1 antibody; p90rsk antibody; p90RSK1 antibody; p90S6K antibody; pp90RSK1 antibody; Ribosomal protein S6 kinase 90kD 1 antibody; Ribosomal protein S6 kinase 90kD polypeptide 1 antibody; Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody; Ribosomal protein S6 kinase alpha 1 antibody; Ribosomal protein S6 kinase alpha-1 antibody; Ribosomal protein S6 kinase polypeptide 1 antibody; Ribosomal S6 kinase 1 antibody; RPS6K1 alpha antibody; rps6ka antibody; Rps6ka1 antibody; RSK 1 antibody; RSK 1 p90 antibody; RSK antibody; RSK-1 antibody; RSK1 antibody; S6K alpha 1 antibody; S6K-alpha-1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.