Phospho-RSK1 p90 (T359 + S363) Recombinant Rabbit Monoclonal Antibody [SU03-65] (ET1608-53)
Catalog# ET1608-53
Rabbit Monoclonal to Phospho-RSK1 p90 (T359+S363)
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-53) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-RSK1 p90 (T359 + S363) Recombinant Rabbit Monoclonal Antibody [SU03-65] (ET1608-53)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr359 and ser363 of human rsk1 p90 aa 341-390 / 735.
Host
Rabbit
Modification
Phospho
Modification Site
T359+S363
Positive Control
HeLa cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, A431, HepG2, 293, human breast carcinoma tissue, mouse liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU03-65
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 83 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-RSK1 p90 (T359+S363)
SYNONYMS
90 kDa ribosomal protein S6 kinase 1 antibody;dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody;dJ590P13.1 antibody;EC 2.7.11.1 antibody;HU 1 antibody;HU1 antibody;KS6A1_HUMAN antibody;MAP kinase activated protein kinase 1a antibody;MAP kinase-activated protein kinase 1a antibody;MAPK-activated protein kinase 1a antibody;MAPKAP kinase 1a antibody;MAPKAPK-1a antibody;MAPKAPK1A antibody;MGC79981 antibody;Mitogen-activated protein kinase-activated protein kinase 1A antibody;OTTHUMP00000004113 antibody;p90 RSK1 antibody;p90-RSK 1 antibody;p90rsk antibody;p90RSK1 antibody;p90S6K antibody;pp90RSK1 antibody;Ribosomal protein S6 kinase 90kD 1 antibody;Ribosomal protein S6 kinase 90kD polypeptide 1 antibody;Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody;Ribosomal protein S6 kinase alpha 1 antibody;Ribosomal protein S6 kinase alpha-1 antibody;Ribosomal protein S6 kinase polypeptide 1 antibody;Ribosomal S6 kinase 1 antibody;RPS6K1 alpha antibody;rps6ka antibody;Rps6ka1 antibody;RSK 1 antibody;RSK 1 p90 antibody;RSK antibody;RSK-1 antibody;RSK1 antibody;S6K alpha 1 antibody;S6K-alpha-1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
CITATIONS
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Zhou Nana,et al
PPP1R14B-mediated phosphorylation enhances protein stability of RPS6KA1 to promote hepatocellular carcinoma tumorigenesis
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WB
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Human
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Citation
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