Phospho-RIP3 (T231+S232) Rabbit Polyclonal Antibody (HA500330)
Catalog# HA500330
Rabbit Polyclonal to Phospho-RIP3 (T231+S232)
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WB
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Mouse
Lane 1: L929 cell lysate
Lane 2: L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 2 hours
Lane 3: L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 3.5 hours
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500330) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Phospho-RIP3 (T231+S232) Rabbit Polyclonal Antibody (HA500330)
Immunogen
Synthetic peptide within mouse rip3 aa 201-250/518.
Host
Rabbit
Modification
Phospho
Modification Site
T231+S232
Positive Control
L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 2 hours, L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 3.5 hours.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-RIP3 (T231+S232)
SYNONYMS
Receptor interacting protein 3 antibody;Receptor interacting serine threonine kinase 3 antibody;Receptor interacting serine/threonine protein kinase 3 antibody;Receptor-interacting protein 3 antibody;Receptor-interacting serine/threonine-protein kinase 3 antibody;RIP 3 antibody;RIP like protein kinase 3 antibody;RIP-3 antibody;RIP-like protein kinase 3 antibody;RIPK 3 antibody;RIPK3 antibody;RIPK3_HUMAN antibody
SUBCELLULAR LOCATION
Cytosol, Nucleus.
FUNCTION
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (By similarity). In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to flavivirus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate.
CITATIONS
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Chen Ying,et al
Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis
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WB
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Mouse
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Citation
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