Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70] (HA722067)
Catalog# HA722067
Rabbit Monoclonal to Phospho-PKR (T451)
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WB
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FC
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IF-Cell
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Human
Western blot analysis of Phospho-PKR (T451) on different lysates with Rabbit anti-Phospho-PKR (T451) antibody (HA722067) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate
Lane 3: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722067) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70] (HA722067)
Immunogen
Synthetic peptide corresponding to residues surrounding thr45 of human pkr protein.
Host
Rabbit
Modification
Phospho
Modification Site
T451
Positive Control
HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, HeLa cells treated with 10ng/mL IFN-α1 for 18 hours then add 100nM Calyculin A for 15 minutes.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE46-70
RRID
APPLICATION DILUTION
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WB
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1:1,000
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FC
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1:1,000
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IF-Cell
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 62 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PKR (T451)
SYNONYMS
Double stranded RNA activated protein kinase; antibody;E2AK2_HUMAN antibody;eIF-2A protein kinase 2 antibody;EIF2AK1 antibody;EIF2AK2 antibody;Eukaryotic translation initiation factor 2 alpha kinase 2 antibody;Eukaryotic translation initiation factor 2-alpha kinase 2 antibody;HGNC:9437 antibody;Interferon induced double stranded RNA activated protein kinase antibody;Interferon inducible elF2 alpha kinase antibody;Interferon inducible RNA dependent protein kinase antibody;Interferon-induced, double-stranded RNA-activated protein kinase antibody;Interferon-inducible RNA-dependent protein kinase antibody;MGC126524 antibody;P1/eIF-2A protein kinase antibody;P1/eIF2A protein kinase antibody;p68 kinase antibody;PKR antibody;PPP1R83 antibody;PRKR antibody;Protein kinase interferon inducible double stranded RNA dependent antibody;Protein kinase RNA activated antibody;Protein kinase RNA-activated antibody;Protein phosphatase 1 regulatory subunit 83 antibody;Serine/threonine protein kinase TIK antibody;Tyrosine protein kinase EIF2AK2 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, perinuclear region.
FUNCTION
Protein kinase RNA-activated also known as protein kinase R (PKR), interferon-induced, double-stranded RNA-activated protein kinase, or eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) is an enzyme that in humans is encoded by the EIF2AK2 gene on chromosome 2. PKR is a serine/tyrosine kinase that is 551 amino acids long. PKR is inducible by various mechanisms of stress and protects against viral infections. It also has a role in several signaling pathways.
CITATIONS
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ADAR1p110 promotes Enterovirus D68 replication through its deaminase domain and inhibition of PKR pathway
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WB
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Human
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Citation
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