Phospho-PKR (T446) Recombinant Rabbit Monoclonal Antibody [SY230] (ET1607-20)
Catalog# ET1607-20
Rabbit Monoclonal to Phospho-PKR (T446)
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WB
-
IHC-P
-
IP
-
IF-Cell
-
Human
Western blot analysis of Phospho-PKR (T446) on different lysates with Rabbit anti-Phospho-PKR (T446) antibody (ET1607-20) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 68 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-20) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PKR (T446) Recombinant Rabbit Monoclonal Antibody [SY230] (ET1607-20)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr446 of human pkr aa 421-470 / 551.
Host
Rabbit
Modification
Phospho
Modification Site
T446
Positive Control
Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Hela treated with Calyculin A and TNF-alpha whole cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human breast carcinoma tissue, human kidney tissue, human small intestine tissue, SiHa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY230
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
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IHC-P
-
1:50-1:200
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IP
-
Use at an assay dependent concentration.
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IF-Cell
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 62 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PKR (T446)
SYNONYMS
Double stranded RNA activated protein kinase; antibody;E2AK2_HUMAN antibody;eIF-2A protein kinase 2 antibody;EIF2AK1 antibody;EIF2AK2 antibody;Eukaryotic translation initiation factor 2 alpha kinase 2 antibody;Eukaryotic translation initiation factor 2-alpha kinase 2 antibody;HGNC:9437 antibody;Interferon induced double stranded RNA activated protein kinase antibody;Interferon inducible elF2 alpha kinase antibody;Interferon inducible RNA dependent protein kinase antibody;Interferon-induced, double-stranded RNA-activated protein kinase antibody;Interferon-inducible RNA-dependent protein kinase antibody;MGC126524 antibody;P1/eIF-2A protein kinase antibody;P1/eIF2A protein kinase antibody;p68 kinase antibody;PKR antibody;PPP1R83 antibody;PRKR antibody;Protein kinase interferon inducible double stranded RNA dependent antibody;Protein kinase RNA activated antibody;Protein kinase RNA-activated antibody;Protein phosphatase 1 regulatory subunit 83 antibody;Serine/threonine protein kinase TIK antibody;Tyrosine protein kinase EIF2AK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
TISSUE SPECIFICITY
Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas, and which correlates with tumor progression and invasiveness or risk of progression.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
An interferon-inducible, RNA-dependent protein serine/threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2 on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and posttranslational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr-446 and Thr-451 in the activation loop are critical for its kinase activity. Thr-446 is the in vivo autophosphorylation site of PKR. Mutation of threonine to alanine at position 446 substantially reduces PKR function, and mutant kinase containing Ala-451 is completely inactive.
