PRODUCT CODE: ET1611-78

Phospho-PKC zeta (T560) Recombinant Rabbit Monoclonal Antibody [SN0753] (ET1611-78)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Phospho-PKC zeta(T560) on mouse eyeball lysates using anti-Phospho-PKC zeta(T560) antibody at 1/1,000 dilution.
  • Western blot analysis of Phospho-PKC zeta(T560) on mouse eyeball lysates using anti-Phospho-PKC zeta(T560) antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.
Western blot analysis of Phospho-PKC zeta(T560) on mouse eyeball lysates using anti-Phospho-PKC zeta(T560) antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-PKC zeta (T560) Recombinant Rabbit Monoclonal Antibody [SN0753] (ET1611-78)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr560 of human pkc zeta.

Host

Rabbit

Modification

Phospho

Modification Site

T560

Positive Control

Human breast carcinoma tissue, mouse brain tissue, mouse eyeball.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN0753

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-PKC zeta (T560)

SYNONYMS

14-3-3-zetaisoform antibody; AI098070 antibody; aPKCzeta antibody; C80388 antibody; EC 2.7.11.13 antibody; KPCZ_HUMAN antibody; nPKC zeta antibody; nPKC-zeta antibody; OTTHUMP00000001368 antibody; OTTHUMP00000044160 antibody; PKC 2 antibody; PKC ZETA antibody; PKC2 antibody; Pkcz antibody; PKCZETA antibody; PKM-zeta, included antibody; PRKCZ antibody; Protein kinase C zeta antibody; Protein kinase C zeta form antibody; Protein kinase C zeta type antibody; r14-3-3 antibody; R74924 antibody; zetaPKC antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

TISSUE SPECIFICITY

Expressed in brain, and to a lesser extent in lung, kidney and testis.

POST-TRANSLATIONAL MODIFICATION

CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.

SUBCELLULAR LOCATION

Cytoplasm, Endosome, Cell junction.

FUNCTION

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, Ω, h, θ, λ/ι, ? and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

CITATIONS

  • Cai, Guodong et al.

    Mechanism and effects of Zearalenone on mouse T lymphocytes activation in vitro. | Ecotoxicology and Environmental Safety [2018]