Phospho-PKC alpha (T638) Recombinant Rabbit Monoclonal Antibody [JF0964] (ET1702-17)
Catalog# ET1702-17
Rabbit Monoclonal to Phospho-PKC alpha (T638)
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Dot Blot
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Human
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Mouse
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Rat
Western blot analysis of Phospho-PKC alpha (T638) on different lysates with Rabbit anti-Phospho-PKC alpha (T638) antibody (ET1702-17) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate(40 µg/Lane)
Lane 6: Rat brain tissue lysate(40 µg/Lane)
Lane 3: HeLa cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-17) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PKC alpha (T638) Recombinant Rabbit Monoclonal Antibody [JF0964] (ET1702-17)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr638 of human pkc alpha aa 622-667 / 672.
Host
Rabbit
Modification
Phospho
Modification Site
T638
Positive Control
HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3 starved overnight then treated with 200nM TPA for 4 hours cell lysate, HeLa, NIH/3T3, C6, human breast cancer tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0964
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:500
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IF-Tissue
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1:50-1:500
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IHC-P
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1:50-1:1,000
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IP
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Use at an assay dependent concentration.
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Dot Blot
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 77 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PKC alpha (T638)
SYNONYMS
AAG6 antibody;Aging associated gene 6 antibody;aPKC antibody;KPCA_HUMAN antibody;PKC alpha antibody;PKC-A antibody;PKC-alpha antibody;PKCA antibody;PRKACA antibody;PRKCA antibody;Protein Kinase C alpha antibody;Protein kinase C alpha type antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane, Mitochondrion membrane, Nucleus.
FUNCTION
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
CITATIONS
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Yongjing Zhang,et al
Corydalis yanhusuo extract and its pharmacological substances alleviate food allergy by inhibiting mast cells activation via PLC/PKC/STAT3 pathway
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WB
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Human
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Citation
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He, J., Lin, M., Zhang, X., Zhang, R., Tian, T., Zhou, Y., Dong, W., Yang, Y., Sun, X., Dai, Y., Xu, Y., Zhang, Z., Xu, M., Lei, Q. Y., Xu, Y., & Lv, L.
TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential
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WB
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Human
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Citation
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Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.
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WB
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Human
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Citation
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