Phospho-PKA R2 (S99) Recombinant Rabbit Monoclonal Antibody [SC54-04] (ET1610-29)
Catalog# ET1610-29
Rabbit Monoclonal to Phospho-PKA R2 (S99)
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
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Pig
Western blot analysis of Phospho-PKA R2 (S99) on different lysates with Rabbit anti-Phospho-PKA R2 (S99) antibody (ET1610-29) at 1/2,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-29) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PKA R2 (S99) Recombinant Rabbit Monoclonal Antibody [SC54-04] (ET1610-29)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser99 of human pka r2 aa 82-126 / 404.
Host
Rabbit
Modification
Phospho
Modification Site
S99
Positive Control
PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human colon cancer tissue, PC-12 cells treated with 100nM Calyculin A for 30 minutes, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC54-04
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:1,000
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IP
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1-2μg/sample
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 46 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PKA R2 (S99)
SYNONYMS
cAMP dependent protein kinase regulatory subunit alpha 2 antibody;cAMP dependent protein kinase regulatory subunit RII alpha antibody;cAMP dependent protein kinase type II alpha regulatory chain antibody;cAMP dependent protein kinase type II alpha regulatory subunit antibody;cAMP-dependent protein kinase type II-alpha regulatory subunit antibody;KAP2 antibody;KAP2_HUMAN antibody;MGC3606 antibody;PKR 2 antibody;PKR2 antibody;PRKA R2 antibody;PRKAR 2 antibody;PRKAR2 antibody;PRKAR2A antibody;Protein kinase A RII alpha subunit antibody;Protein kinase cAMP dependent regulatory type II alpha antibody
SEQUENCE SIMILARITIES
Belongs to the cAMP-dependent kinase regulatory chain family.
TISSUE SPECIFICITY
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by the activated catalytic chain.
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm.
FUNCTION
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
CITATIONS
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Koda, S., Zhang, B., Zhou, Q. Y., Xu, N., Li, J., Liu, J. X., Liu, M., Lv, Z. Y., Wang, J. L., Shi, Y., Gao, S., Yu, Q., Li, X. Y., Xu, Y. H., Chen, J. X., Tekengne, B., Adzika, G. K., Tang, R. X., Sun, H., Zheng, K. Y., … Yan, C.
β2-Adrenergic Receptor Enhances the Alternatively Activated Macrophages and Promotes Biliary Injuries Caused by Helminth Infection
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WB
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Mouse
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Citation
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