Phospho-PDGFR beta (Y1009) Recombinant Rabbit Monoclonal Antibody [JE46-72] (HA721736)
Catalog# HA721736
Rabbit Monoclonal to Phospho-PDGFR beta (Y1009)
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 200 kDa (Glycosylation)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 200 kDa (Glycosylation)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.