Positive control:
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 2: SH-SY5Y cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Recombinant Rabbit Monoclonal Antibody [SU03-70] (ET1608-58)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser144 of human pak1.
Host
Rabbit
Modification
Phospho
Modification Site
PAK1(S144)+PAK2(S141)+PAK3(S139)
Positive Control
Hela cell lysate, NIH/3T3 cell lysate, SH-SY5Y cell lysate, Hela, NIH/3T3, human liver carcinoma tissue, human colon carcinoma tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU03-70
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
65 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139)
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
TISSUE SPECIFICITY
Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated when activated by CDC42/p21.; Neddylated.
SUBCELLULAR LOCATION
Cell membrane, ruffle membrane, nucleoplasm, Cytoplasm, focal adhesion, invadopodium, Chromosome.
FUNCTION
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.