Phospho-p70 S6 Kinase (T389) Recombinant Rabbit Monoclonal Antibody [PSH02-23] (HA721803)
Catalog# HA721803
Rabbit Monoclonal to Phospho-P70 S6 Kinase (T389)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of Phospho-p70 S6 Kinase (T389) on different lysates with Rabbit anti-Phospho-p70 S6 Kinase (T389) antibody (HA721803) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 70/85 kDa
Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721803) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-p70 S6 Kinase (T389) Recombinant Rabbit Monoclonal Antibody [PSH02-23] (HA721803)
Immunogen
Synthetic peptide within human p70 s6 kinase aa 371-420 / 525.
Host
Rabbit
Modification
Phospho
Modification Site
T389
Positive Control
MCF7 cell lysate, MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, human breast carcinoma tissue, MCF7 starved for 4 hours then treated with 200ng/mL EGF for 15 minutes cell lysate, C6 cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human liver carcinoma tissue, 293T cell lysate, 293T serum starved overnight cell lysate, 293T serum starved overnight then treated with 100nM Insulin for 15 minutes cell lysate, MCF7 cells treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH02-23
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 59 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-P70 S6 Kinase (T389)
SYNONYMS
70 kDa ribosomal protein S6 kinase 1 antibody;p70 alpha antibody;p70 S6 kinase alpha antibody;p70 S6K antibody;p70 S6KA antibody;p70(S6K) alpha antibody;p70s6kA antibody;PS6K antibody;Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody;ribosomal protein S6 kinase beta 1 antibody;ribosomal protein S6 kinase I antibody;Ribosomal protein S6 kinase, 70-kD antibody;RPS6KB1 antibody;S6K antibody;S6K1 antibody;Serine/threonine kinase 14 alpha antibody;Serine/threonine protein kinase 14A antibody;STK14A antibody
SUBCELLULAR LOCATION
Synapse, synaptosome, Mitochondrion outer membrane, Mitochondrion.
FUNCTION
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
CITATIONS
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Zhiruo Wang ,et al
Zotarolimus alleviates post-trabeculectomy fibrosis via dual functions of anti-inflammation and regulating AMPK/mTOR axis
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IF
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Citation
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Mettl14 sustains FOXP3 expression to promote the differentiation and functions of induced-regulatory T cells via the mTOR signaling pathway
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WB
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Mouse
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Citation
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