PRODUCT CODE: ER2001-51

Phospho-p38 (T180) Rabbit Polyclonal Antibody (ER2001-51)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of p38  (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293 cell lysate, untreated with UV<br />
Lane 2: 293 cell lysate, treated with UV for 60 minutes
  • Western blot analysis of p38  (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293 cell lysate, untreated with UV<br />
Lane 2: 293 cell lysate, treated with UV for 60 minutes
  • Western blot analysis of p38  (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate, untreated with UV<br />
Lane 2: A431 cell lysate, treated with UV for 40 minutes
  • Western blot analysis of p38  (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate, untreated<br />
Lane 2: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes <br />
Lane 3: Hela cell lysate, treated with UV for 40 minutes
Western blot analysis of p38 (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate, untreated with UV
Lane 2: 293 cell lysate, treated with UV for 60 minutes

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Phospho-p38 (T180) Rabbit Polyclonal Antibody (ER2001-51)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr180 of human p38.

Host

Rabbit

Modification

Phospho

Modification Site

T180

Positive Control

293 cell lysate untreated with UV, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate untreated with UV, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes, Hela cell lysate treated with UV for 40 minutes.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Phospho-p38 (T180)

SYNONYMS

CSAID Binding Protein 1 antibody; CSAID binding protein antibody; CSAID-binding protein antibody; Csaids binding protein antibody; CSBP 1 antibody; CSBP 2 antibody; CSBP antibody; CSBP1 antibody; CSBP2 antibody; CSPB1 antibody; Cytokine suppressive anti-inflammatory drug-binding protein antibody; EXIP antibody; MAP kinase 14 antibody; MAP kinase MXI2 antibody; MAP kinase p38 alpha antibody; MAPK 14 antibody; MAPK14 antibody; MAX interacting protein 2 antibody; MAX-interacting protein 2 antibody; Mitogen Activated Protein Kinase 14 antibody; Mitogen activated protein kinase p38 alpha antibody; Mitogen-activated protein kinase 14 antibody; Mitogen-activated protein kinase p38 alpha antibody; MK14_HUMAN antibody; Mxi 2 antibody; MXI2 antibody; p38 ALPHA antibody; p38 antibody; p38 MAP kinase antibody; p38 MAPK antibody; p38 mitogen activated protein kinase antibody; p38ALPHA antibody; p38alpha Exip antibody; PRKM14 antibody; PRKM15 antibody; RK antibody; SAPK2A antibody; Stress-activated protein kinase 2a antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.