Phospho-p38 (T180) Recombinant Rabbit Monoclonal Antibody [JE77-37] (HA722150)
Catalog# HA722150
Rabbit Monoclonal to Phospho-p38 (T180)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of Phospho-p38 (T180) on different lysates with Rabbit anti-Phospho-p38 (T180) antibody (HA722150) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with UV for 1 hour cell lysate
Lane 3: Jurkat treated with UV for 1 hour cell lysate, then the membrane treated with λpp for 1 hour
Lane 4: PC-12 cell lysate
Lane 5: PC-12 treated with UV for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722150) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-p38 (T180) Recombinant Rabbit Monoclonal Antibody [JE77-37] (HA722150)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding thr180 of human p38 mapk.
Host
Rabbit
Modification
Phospho
Modification Site
T180
Positive Control
Jurkat cell lysate, Jurkat treated with UV for 1 hour cell lysate, PC-12 cell lysate, PC-12 treated with UV for 1 hour cell lysate, Jurkat cells treated with UV for 1 hour, human breast cancer tissue, mouse colon tissue, rat colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE77-37
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-p38 (T180)
SYNONYMS
CSAID Binding Protein 1 antibody;CSAID binding protein antibody;CSAID-binding protein antibody;Csaids binding protein antibody;CSBP 1 antibody;CSBP 2 antibody;CSBP antibody;CSBP1 antibody;CSBP2 antibody;CSPB1 antibody;Cytokine suppressive anti-inflammatory drug-binding protein antibody;EXIP antibody;MAP kinase 14 antibody;MAP kinase MXI2 antibody;MAP kinase p38 alpha antibody;MAPK 14 antibody;MAPK14 antibody;MAX interacting protein 2 antibody;MAX-interacting protein 2 antibody;Mitogen Activated Protein Kinase 14 antibody;Mitogen activated protein kinase p38 alpha antibody;Mitogen-activated protein kinase 14 antibody;Mitogen-activated protein kinase p38 alpha antibody;MK14_HUMAN antibody;Mxi 2 antibody;MXI2 antibody;p38 ALPHA antibody;p38 antibody;p38 MAP kinase antibody;p38 MAPK antibody;p38 mitogen activated protein kinase antibody;p38ALPHA antibody;p38alpha Exip antibody;PRKM14 antibody;PRKM15 antibody;RK antibody;SAPK2A antibody;Stress-activated protein kinase 2a antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
p38 mitogen-activated protein kinases are a class of mitogen-activated protein kinases (MAPKs) that are responsive to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock, and are involved in cell differentiation, apoptosis and autophagy. Persistent activation of the p38 MAPK pathway in muscle satellite cells (muscle stem cells) due to ageing, impairs muscle regeneration. p38 MAP Kinase (MAPK), also called RK or CSBP (Cytokinin Specific Binding Protein), is the mammalian orthologue of the yeast Hog1p MAP kinase, which participates in a signaling cascade controlling cellular responses to cytokines and stress.
CITATIONS
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Li Xinhao,et al
The Link between Osteoporosis and Frozen Shoulder: Exploring the Therapeutic Effect of TAK715 on Reversing Fibrosis and Protecting against Osteoporosis via the p38 MAPK Signaling Pathway
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WB
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Human
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Citation
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Wang Jinyang,et al
Edaravone Dexborneol ameliorates the cognitive deficits of APP/PS1 mice by inhibiting TLR4/MAPK signaling pathway via upregulating TREM2
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WB
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Mouse
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Citation
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