Phospho-Nucleophosmin (T199) Recombinant Rabbit Monoclonal Antibody [JE63-16] (HA722125)
Catalog# HA722125
Rabbit Monoclonal to Phospho-Nucleophosmin (T199)
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Nucleophosmin (T199) on different lysates with Rabbit anti-Phospho-Nucleophosmin (T199) antibody (HA722125) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 38 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Nucleophosmin (T199) on different lysates with Rabbit anti-Phospho-Nucleophosmin (T199) antibody (HA722125) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 38 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.