Phospho-Nucleolin (T84) Recombinant Rabbit Monoclonal Antibody [JE64-18] (HA722920)
Catalog# HA722920
Rabbit Monoclonal to Phospho-Nucleolin (T84)
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-Nucleolin (T84) on different lysates with Rabbit anti-Phospho-Nucleolin (T84) antibody (HA722920) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 105 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722920) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 105 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722920) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.