Phospho-Nucleolin (T76) Recombinant Rabbit Monoclonal Antibody [JE64-03] (HA722942)
Catalog# HA722942
Rabbit Monoclonal to Phospho-Nucleolin (T76)
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Nucleolin (T76) on different lysates with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate (20 µg/Lane)
Lane 3: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 77 kDa
Observed band size: 105 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722942) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Nucleolin (T76) on different lysates with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate (20 µg/Lane)
Lane 3: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 77 kDa
Observed band size: 105 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722942) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.