Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64] (HA721737)
Catalog# HA721737
Rabbit Monoclonal to Phospho-MST1/MST2 (T180 + T183)
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WB
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Human
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Mouse
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Rat
Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64] (HA721737)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding thr183 of human mst1 protein.
Host
Rabbit
Modification
Phospho
Modification Site
T180 + T183
Positive Control
Ramos treated with 1μM Staurosporine for 1 hour cell lysate, NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate, C6 treated with 1μM Staurosporine for 3 hour cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE46-64
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 56 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-MST1/MST2 (T180 + T183)
SYNONYMS
DKFZp686A2068 antibody;FLJ90748 antibody;KRS1 antibody;KRS2 antibody;Mammalian STE20 like protein kinase 1 antibody;Mammalian STE20 like protein kinase 2 antibody;MST 1 antibody;MST 2 antibody;MST1 antibody;MST2 antibody;Serine/threonine kinase 3 (STE20 homolog yeast) antibody;Serine/threonine kinase 3 (Ste20 yeast homolog) antibody;Serine/threonine kinase 3 antibody;Serine/threonine kinase 4 antibody;Serine/threonine protein kinase 3 antibody;Serine/threonine protein kinase 4 antibody;Serine/threonine protein kinase Krs 1 antibody;Serine/threonine protein kinase Krs 2 antibody;STE20 like kinase MST1 antibody;STE20 like kinase MST2 antibody;STK 3 antibody;STK 4 antibody;STK3 antibody;STK4 antibody;YSK3 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
CITATIONS
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Zi-qiong Wang ,et al
Cyclovirobuxine D Inhibits Triple-Negative Breast Cancer via YAP/TAZ Suppression and Activation of the FOXO3a/PINK1-Parkin Pathway-Induced Mitophagy
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WB
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Human
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Citation
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