Phospho-MLKL (S345) Recombinant Rabbit Monoclonal Antibody [JM92-37] (ET1705-51)
Catalog# ET1705-51
Rabbit Monoclonal to Phospho-MLKL (S345)
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WB
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IHC-P
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IHC-Fr
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Mouse
Western blot analysis of Phospho-MLKL (S345) on different lysates with Rabbit anti-Phospho-MLKL (S345) antibody (ET1705-51) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: L-929 cell lysate
Lane 2: L929 treated with 20μM Z-VAD for 3.5 hours, then added 100nM SM-164 and 20ng/ml TNF-α for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-51) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-MLKL (S345) Recombinant Rabbit Monoclonal Antibody [JM92-37] (ET1705-51)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser345 of mouse mlkl.
Host
Rabbit
Modification
Phospho
Modification Site
S345
Positive Control
L929 treated with 20μM Z-VAD for 3.5 hours then added 100nM SM-164 and 20ng/ml TNF-α for 3 hours cell lysate, mouse spleen tissue, mouse lung tissue, mouse liver tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM92-37
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:100
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IHC-Fr
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-MLKL (S345)
SYNONYMS
9130019I15Rik antibody;FLJ34389 antibody;hMLKL antibody;Mixed lineage kinase domain like antibody;Mixed lineage kinase domain like protein antibody;Mixed lineage kinase domain like pseudokinase antibody;Mixed lineage kinase domain-like protein antibody;Mlkl antibody;MLKL_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily.
TISSUE SPECIFICITY
Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm, Nucleus.
FUNCTION
Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
CITATIONS
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He Ruobing,et al
Aurantiamide mitigates acute kidney injury by suppressing renal necroptosis and inflammation via GRPR-dependent mechanism
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IF
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WB
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Human
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Citation
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Tojo, K., Yamamoto, N., Tamada, N., Mihara, T., Abe, M., Nishii, M., Takeuchi, I., & Goto, T.
Early alveolar epithelial cell necrosis is a potential driver of COVID-19-induced acute respiratory distress syndrome
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IHC-P
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Mouse
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Citation
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Chen Ying,et al
Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis
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WB
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Mouse
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Citation
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Lin Yihuang,et al
Age-related dysregulation of CXCL9/10 in monocytes is linked to impaired innate immune responses in a mouse model of Staphylococcus aureus osteomyelitis
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IHC
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Citation
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Zhnag Qi,et al
The cGAS/STING signaling pathway is involved in sevoflurane induced neuronal necroptosis via regulating microglia M1 polarization
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WB
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Mouse
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Citation
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