Phospho-Met (Y1349) Recombinant Rabbit Monoclonal Antibody [JE51-68] (ET7110-14)

UNIPROT #

P08581

PROTEIN NAME

Phospho-Met (pY1349)

SYNONYMS

AUTS9 antibody;c met antibody;D249 antibody;Hepatocyte growth factor receptor antibody;HGF antibody;HGF receptor antibody;HGF/SF receptor antibody;HGFR antibody;MET antibody;Met proto oncogene tyrosine kinase antibody;MET proto oncogene, receptor tyrosine kinase antibody;Met proto-oncogene (hepatocyte growth factor receptor) antibody;Met proto-oncogene antibody;Met protooncogene antibody;MET_HUMAN antibody;Oncogene MET antibody;Par4 antibody;Proto-oncogene c-Met antibody;RCCP2 antibody;Scatter factor receptor antibody;SF receptor antibody;Tyrosine-protein kinase Met antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family.

TISSUE SPECIFICITY

Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level).

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2.; Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors.; (Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified.

SUBCELLULAR LOCATION

Membrane, secreted.

FUNCTION

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).