Phospho-MCM2 (S41) Recombinant Rabbit Monoclonal Antibody [JE50-04] (HA722279)
Catalog# HA722279
Rabbit Monoclonal to Phospho-MCM2 (S41)
Tested for
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WB
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IF-Cell
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IHC-P
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-MCM2 (S41) on different lysates with Rabbit anti-Phospho-MCM2 (S41) antibody (HA722279) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (30 µg/Lane)
Lane 6: Mouse thymus tissue lysate (30 µg/Lane)
Lane 7: Rat spleen tissue lysate (30 µg/Lane)
Lane 8: Rat thymus tissue lysate (30 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-4: 8 seconds; Lane 5-8: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (30 µg/Lane)
Lane 6: Mouse thymus tissue lysate (30 µg/Lane)
Lane 7: Rat spleen tissue lysate (30 µg/Lane)
Lane 8: Rat thymus tissue lysate (30 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-4: 8 seconds; Lane 5-8: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.