Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-43] (HA751290)
Catalog# HA751290
Rabbit Monoclonal to Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389)
Tested for
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WB
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution.
Lane 1: COLO205 cell lysate
Lane 2: TT cell lysate (negative)
Lane 3: Ramos serum starved for 16 hours cell lysate
Lane 4: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lane 5: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution.
Lane 1: COLO205 cell lysate
Lane 2: TT cell lysate (negative)
Lane 3: Ramos serum starved for 16 hours cell lysate
Lane 4: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lane 5: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.