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Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221)) Recombinant Rabbit Monoclonal Antibody [ST500] (ET1609-42)

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Catalog# ET1609-42

Rabbit Monoclonal to Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221))

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

React with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221)) Recombinant Rabbit Monoclonal Antibody [ST500] (ET1609-42)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr183 + thr183 + thr221 of human jnk1 / 2 / 3 aa 161-204 / 427.

Host

Rabbit

Modification

Phospho

Modification Site

JNK1(T183)+JNK2(T183)+JNK3(T221)

Positive Control

NIH/3T3 cell lysate treated with Anisomycin, Hela cell lysate treated with Anisomycin, A431 cell lysate treated with UV40, 293 cell lysate treated with UV40, Hela cell lysate treated with UV40, Hela, NIH/3T3, HUVEC, human colon tissue, human endometrium tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST500

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:400

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 48/53 kDa

Isotype

IgG

TARGET

UNIPROT #

P45983

PROTEIN NAME

Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221))

SYNONYMS

C Jun kinase 2 antibody;c Jun N terminal kinase 1 antibody;c Jun N terminal kinase 2 antibody;c Jun N terminal kinase 3 antibody;c-Jun N-terminal kinase 1 antibody;JNK 46 antibody;JNK 55 antibody;JNK antibody;JNK-46 antibody;JNK1 antibody;JNK1A2 antibody;JNK2 antibody;JNK21B1/2 antibody;JNK2A antibody;JNK2ALPHA antibody;JNK2B antibody;JNK2BETA antibody;JNK3 alpha protein kinase antibody;JNK3 antibody;JNK3A antibody;Jun kinase antibody;JUN N terminal kinase antibody;MAP kinase 10 antibody;MAP kinase 8 antibody;MAP kinase 9 antibody;MAP kinase p49 3F12 antibody;MAPK 10 antibody;MAPK 8 antibody;MAPK 9 antibody;MAPK10 antibody;mapk8 antibody;MAPK9 antibody;Mitogen activated protein kinase 10 antibody;Mitogen activated protein kinase 8 antibody;Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody;Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody;Mitogen activated protein kinase 9 antibody;Mitogen-activated protein kinase 8 antibody;MK08_HUMAN antibody;p493F12 antibody;p54a antibody;p54aSAPK antibody;p54bSAPK antibody;PRKM10 antibody;PRKM8 antibody;PRKM9 antibody;SAPK antibody;SAPK(beta) antibody;SAPK1 antibody;SAPK1a antibody;SAPK1b antibody;SAPK1c antibody;Stress activated protein kinase 1 antibody;Stress activated protein kinase 1a antibody;Stress activated protein kinase 1b antibody;Stress activated protein kinase 1c antibody;Stress activated protein kinase beta antibody;Stress activated protein kinase JNK1 antibody;Stress activated protein kinase JNK2 antibody;Stress activated protein kinase JNK3 antibody;Stress-activated protein kinase 1c antibody;Stress-activated protein kinase JNK1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Membrane, Mitochondrion, Nucleus.

FUNCTION

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

CITATIONS

  • Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.

    Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota

  • Li, P., Yang, Y., Lin, Z., Hong, S., Jiang, L., Zhou, H., Yang, L., Zhu, L., Liu, X., & Liu, L.

    Bile Duct Ligation Impairs Function and Expression of Mrp1 at Rat Blood-Retinal Barrier via Bilirubin-Induced P38 MAPK Pathway Activations

  • Li, H., Ou, G., He, Y., Ren, L., Yang, X., & Zeng, M.

    Resveratrol attenuates the MSU crystal-induced inflammatory response through the inhibition of TAK1 activity. International immunopharmacology, 67, 62–68.

  • Chao Shen

    Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling

  • Wu, H., Guo, H., Liu, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L.

    Copper sulfate-induced endoplasmic reticulum stress promotes hepatic apoptosis by activating CHOP, JNK and caspase-12 signaling pathways

  • Xiawei Wei

    Napabucasin,a novel inhibitor of STAT3,inhibits growth and synergises with doxorubicin in diffuse large B-cell lymphoma

  • Gong, Y., Qiu, J., Ye, J., Jiang, T., Zhang, W., Zheng, X., Zhu, Z., Chen, L., Wang, Z., Mi, S., & Hong, Z.

    AZ-628 delays osteoarthritis progression via inhibiting the TNF-α-induced chondrocyte necroptosis and regulating osteoclast formation

  • Yuan, Y., Wang, X., Li, J., Han, L., Du, H., Sun, Y., Yang, P., Zhou, Z., Gu, M., Lu, Y., & Shen, C.

    Single-Cell Sequencing Yields Insights in the Evolution of Foot-and-Mouth Disease Virus Persistent Infection

  • Yin, Y., Wang, J., Xu, X., Zhou, B., Chen, S., Qin, T., & Peng, D.

    Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine

  • Shi, H., Yang, D., Huo, Z., Li, Y., Guo, W., & Zhang, S.

    MG132 protects against lung injury following brain death in rats

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