Phospho-JAK2 (Y1007+Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03] (ET1607-34)
Catalog# ET1607-34
Rabbit Monoclonal to Phospho-JAK2 (Y1007+Y1008)
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 120 kDa
Exposure time: 3 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-34) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-JAK2 (Y1007+Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03] (ET1607-34)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding tyr1007 and 1008 of human jak2.
Host
Rabbit
Modification
Phospho
Modification Site
Y1007+Y1008
Positive Control
HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, human lung carcinoma tissue, human tonsil tissue, human kidney tissue, rat kidney tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY24-03
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:200-1:500
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 131 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-JAK2 (Y1007+Y1008)
SYNONYMS
JAK 2 antibody;JAK-2 antibody;JAK2 antibody;JAK2_HUMAN antibody;Janus Activating Kinase 2 antibody;Janus kinase 2 (a protein tyrosine kinase) antibody;Janus kinase 2 antibody;JTK 10 antibody;JTK10 antibody;kinase Jak2 antibody;OTTHUMP00000043260 antibody;THCYT3 antibody;Tyrosine protein kinase JAK2 antibody;Tyrosine-protein kinase JAK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
TISSUE SPECIFICITY
Ubiquitously expressed throughout most tissues.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.
SUBCELLULAR LOCATION
Nucleus, Endomembrane system, Cytoplasm.
FUNCTION
JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immuno-logical stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions.
CITATIONS
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Wang Yingying,et al
Linderae Radix extract attenuates ulcerative colitis by inhibiting the JAK/STAT signaling pathway
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WB
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IHC
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Mouse
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Citation
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Gu, X., Miao, Z., Wang, Y., Yang, Y., Yang, T., & Xu, Y.
New Baitouweng decoction combined with fecal microbiota transplantation alleviates DSS-induced colitis in rats by regulating gut microbiota metabolic homeostasis and the STAT3/NF-κB signaling pathway
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WB
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Rat
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Citation
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Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.
Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways
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WB
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Mouse
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Citation
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Zhang Jie,et al
Cediranib enhances the transcription of MHC-I by upregulating IRF-1
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WB
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Human
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Mouse
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Citation
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Novel synthetic 4-chlorobenzoyl berbamine inhibits c-Myc expression and induces apoptosis of diffuse large B cell lymphoma cells.
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WB
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Human
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Citation
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Liu, Y., Hao, Y., Zhao, H., Zhang, Y., Cheng, D., Zhao, L., Peng, Y., Lu, Y., & Li, Y.
PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells
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WB
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Human
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Citation
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Deng, L., Zhang, X., Xiang, X., Xiong, R., Xiao, D., Chen, Z., Liu, K., & Feng, G.
NANOG Promotes Cell Proliferation, Invasion, and Stemness via IL-6/STAT3 Signaling in Esophageal Squamous Carcinoma
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WB
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Human
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Citation
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Li Weijing,et al
Dexmedetomidine hydrochloride plus sufentanil citrate inhibits glucose metabolism and epithelial‑mesenchymal transition in human esophageal squamous carcinoma KYSE30 cells by modulating the JAK/STAT3/HIF‑1α axis
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WB
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Human
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Citation
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Fu, Y., Peng, Y., Zhao, S., Mou, J., Zeng, L., Jiang, X., Yang, C., Huang, C., Li, Y., Lu, Y., Wu, M., Yang, Y., Kong, T., Lai, Q., Wu, Y., Yao, Y., Wang, Y., Gou, L., & Yang, J.
Combination Foretinib and Anti-PD-1 Antibody Immunotherapy for Colorectal Carcinoma. Frontiers in cell and developmental biology, 9, 689727.
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WB
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Mouse
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Citation
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IL-4/IL-13 upregulates Sonic hedgehog expression to induce allergic airway epithelial remodeling
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WB
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Mouse
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Citation
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Acute myeloid leukemia (AML)‐derived mesenchymal stem cells induce chemoresistance and epithelial–mesenchymal transition‐like program in AML through IL‐6/JAK2/STAT3 signaling
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WB
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Human
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Citation
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Li, Y., Guo, F., Huang, R., Ma, L., & Fu, P.
Natural flavonoid pectolinarigenin alleviated kidney fibrosis via inhibiting the activation of TGFβ/SMAD3 and JAK2/STAT3 signaling. International immunopharmacology, 91, 107279.
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WB
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Mouse
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Citation
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Ren, Q., Tao, S., Guo, F., Wang, B., Yang, L., Ma, L., & Fu, P.
Natural flavonol fisetin attenuated hyperuricemic nephropathy via inhibiting IL-6/JAK2/STAT3 and TGF-β/SMAD3 signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology, 87, 153552.
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WB
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IHC
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Mouse
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Citation
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Yang Boshen,et al
Ruxolitinib-based senomorphic therapy mitigates cardiomyocyte senescence in septic cardiomyopathy by inhibiting the JAK2/STAT3 signaling pathway
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WB
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Mouse
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Citation
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