Phospho-IKB alpha (S36) Recombinant Rabbit Monoclonal Antibody [JE56-65] (HA721802)

UNIPROT #

Q9Z1E3

PROTEIN NAME

Phospho-IKB alpha (S36)

SYNONYMS

I kappa B alpha antibody;I-kappa-B-alpha antibody;IkappaBalpha antibody;IkB-alpha antibody;IKBA antibody;IKBA_HUMAN antibody;IKBalpha antibody;MAD 3 antibody;MAD3 antibody;Major histocompatibility complex enhancer-binding protein MAD3 antibody;NF kappa B inhibitor alpha antibody;NF-kappa-B inhibitor alpha antibody;NFKBI antibody;NFKBIA antibody;Nuclear factor of kappa light chain gene enhancer in B cells antibody;Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

SEQUENCE SIMILARITIES

Belongs to the NF-kappa-B inhibitor family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.; Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.; Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.; Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.