PRODUCT CODE: ET1611-19

Phospho-Hormone sensitive lipase (S853) Recombinant Rabbit Monoclonal Antibody [SN06-39] (ET1611-19)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of phospho -Hormone sensitive lipase (S853) on human skeletal muscle lysates using anti- phospho -Hormone sensitive lipase (S853) antibody at 1/1,000 dilution.
  • Western blot analysis of phospho -Hormone sensitive lipase (S853) on human skeletal muscle lysates using anti- phospho -Hormone sensitive lipase (S853) antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Phospho-Hormone sensitive lipase (S853) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Hormone sensitive lipase (S853) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of phospho -Hormone sensitive lipase (S853) on human skeletal muscle lysates using anti- phospho -Hormone sensitive lipase (S853) antibody at 1/1,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-Hormone sensitive lipase (S853) Recombinant Rabbit Monoclonal Antibody [SN06-39] (ET1611-19)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Phospho

Modification Site

S853

Positive Control

Human skeletal muscle lysates, human fetal skeletal muscle tissue, mouse skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN06-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

Phospho-Hormone sensitive lipase (S853)

SYNONYMS

Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS antibody; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the 'GDXG' lipolytic enzyme family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by AMPK may block translocation to lipid droplets.

SUBCELLULAR LOCATION

Cell membrane, cytosol, caveola, Lipid droplet.

FUNCTION

HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.