Phospho-Hormone sensitive lipase (S853) Recombinant Rabbit Monoclonal Antibody [SN06-39] (ET1611-19)
Catalog# ET1611-19
Rabbit Monoclonal to Phospho-Hormone sensitive lipase (S853)
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WB
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of Phospho-Hormone sensitive lipase (S853) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/1,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lane 3: Rat skeletal muscle tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 30 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 84 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-19) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Hormone sensitive lipase (S853) Recombinant Rabbit Monoclonal Antibody [SN06-39] (ET1611-19)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser853 of human lipe aa 821-870 / 1,076.
Specificity
Phospho-hsl (s853) [sn06-39] antibody detects endogenous levels of hsl only when phosphorylated at ser853 by human hsl protein. this antibody cross-react with ser557 phosphorylated mouse hsl protein and ser563 phosphorylated rat hsl protein.
Host
Rabbit
Modification
Phospho
Modification Site
S853
Positive Control
Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human fetal skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN06-39
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 117 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Hormone sensitive lipase (S853)
SYNONYMS
Hormone sensitive lipase antibody;Hormone sensitive lipase testicular isoform antibody;Hormone-sensitive lipase antibody;HSL antibody;LHS antibody;Lipase hormone sensitive antibody;LIPE antibody;LIPS_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the 'GDXG' lipolytic enzyme family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation by AMPK may block translocation to lipid droplets.
SUBCELLULAR LOCATION
Cell membrane, cytosol, caveola, Lipid droplet.
FUNCTION
HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.
CITATIONS
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Melatonin inhibits testosterone synthesis in Roosters Leydig cells by regulating lipolysis of lipid droplets
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WB
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Chicken
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Citation
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