PRODUCT CODE: ET7110-10

Phospho-HER3/ErbB3 (pY1222) Recombinant Rabbit Monoclonal Antibody [JE51-89] (ET7110-10)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

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Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-HER3/ErbB3 (pY1222) Recombinant Rabbit Monoclonal Antibody [JE51-89] (ET7110-10)

Immunogen

Synthetic phosphopeptide corresponding to a region surrounding tyrosine 1222 of human erbb3.

Host

Rabbit

Modification

Phospho

Modification Site

pY1222

Positive Control

Human thyroid tissue, human skin tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE51-89

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

Predicted band size: 148 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-HER3/ErbB3 (pY1222)

SYNONYMS

c erbB 3 antibody; c erbB3 antibody; Erb b2 receptor tyrosine kinase 3 antibody; ErbB 3 antibody; ERBB3 antibody; ERBB3 protein antibody; erbB3 S antibody; ERBB3_HUMAN antibody; Glial growth factor receptor antibody; HER 3 antibody; HER3 antibody; Human epidermal growth factor receptor 3 antibody; LCCS2 antibody; MDA BF 1 antibody; MGC88033 antibody; p180 ErbB3 antibody; p45 sErbB3 antibody; p85 sErbB3 antibody; proto-oncogene-like protein c ErbB 3 antibody; proto-oncogene-like protein c ErbB3 antibody; Proto-oncogene-like protein c-ErbB-3 antibody; Receptor tyrosine protein kinase erbB 3 antibody; Receptor tyrosine protein kinase erbB3 antibody; Receptor tyrosine-protein kinase erbB-3 antibody; Tyrosine kinase type cell surface receptor HER3 antibody; Tyrosine kinase-type cell surface receptor HER3 antibody; v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody; v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody; v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

TISSUE SPECIFICITY

Epithelial tissues and brain.

DEVELOPMENTAL STAGE

Overexpressed in a subset of human mammary tumors.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.

SUBCELLULAR LOCATION

Cell membrane, secreted.

FUNCTION

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.