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Phospho-GSK3 beta (S9) Recombinant Rabbit Monoclonal Antibody [SY02-71] (ET1607-60)

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Catalog# ET1607-60

Rabbit Monoclonal to Phospho-GSK3 beta (S9)

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-GSK3 beta (S9) Recombinant Rabbit Monoclonal Antibody [SY02-71] (ET1607-60)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser9 of human gsk3 beta aa 1-50 / 420.

Host

Rabbit

Modification

Phospho

Modification Site

S9

Positive Control

HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate, C6 cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, C6 treated with 150nM insulin for 15 minutes cell lysate, HeLa cells treated with 100nM Calyculin A for 30 minutes, NIH/3T3 cells treated with 100ng/mL PDGF for 5 minutes, C6 cells treated with 100ng/mL PDGF for 5 minutes, human colon carcinoma tissue, human breast tissue, human breast carcinoma tissue, human kidney tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY02-71

RRID

AB_3069776

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 47 kDa

Isotype

IgG

TARGET

UNIPROT #

P49841

PROTEIN NAME

Phospho-GSK3 beta (S9)

SYNONYMS

Glycogen Synthase Kinase 3 Beta antibody;Glycogen synthase kinase-3 beta antibody;GSK 3 beta antibody;GSK-3 beta antibody;GSK3B antibody;GSK3B_HUMAN antibody;GSK3beta isoform antibody;Serine/threonine-protein kinase GSK3B antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.

TISSUE SPECIFICITY

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216. Inactivated by phosphorylation at Ser-9 (Probable). Phosphorylated in a circadian manner in the hippocampus (By similarity).; Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane.

FUNCTION

Glycogen synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation.

CITATIONS

  • FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors

  • Yangfan Liu , Yu Sun , Jin Yang ,et al

    DNMT1-targeting remodeling global DNA hypomethylation for enhanced tumor suppression and circumvented toxicity in oral squamous cell carcinoma

  • Yun, X., Fang, Y., Lv, C., Qiao, S., Tao, Y., Dai, Y., & Xia, Y.

    Inhibition of the activation of γδT17 cells throughPPARγ–PTEN/Akt/GSK3β/NFAT pathwaycontributes to the anti-colitis effect of madecassicacid

  • Jian Li; Caiqiao Zhang

    Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways

  • Junhao Huang,et al

    The volatile oil of Acorus tatarinowii Schott ameliorates Alzheimer's disease through improving insulin resistance via activating the PI3K/AKT pathway

  • Dong, J., Guo, C., Yang, Z., Wu, Y., & Zhang, C.

    Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens

  • Shang, K., Ma, N., Che, J., Li, H., Hu, J., Sun, H., & Cao, B.

    SLC27A2 mediates FAO in colorectal cancer through nongenic crosstalk regulation of the PPARs pathway

  • Wu Sichen,et al

    Icariin, a natural flavonoid glucoside, inhibits neuroinflammation in mice with triple-transgenic Alzheimer's disease by regulating the Akt/GSK-3β signaling pathway

  • Li Zong,et al

    Schisandrin A alleviates non-alcoholic fatty liver disease by improving liver metabolic disorders and suppressing the GSK3β signaling pathway

  • Guodong Chen;Longquan Shao

    Rapamycin-Induced Autophagy Promotes the Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells in the Temporomandibular Joint in Response to IL-1β

  • Haoyan Wu,et al

    A Multifunctional miRNA Delivery System Based on Tetrahedral Framework Nucleic Acids for Regulating Inflammatory Periodontal Ligament Stem Cells and Attenuating Periodontitis Bone Loss

  • Li Caixia,et al

    Fucoidan ameliorates diabetic skeletal muscle atrophy through PI3K/Akt pathway

  • Xue, F., Zhao, Z., Gu, Y., Han, J., Ye, K., & Zhang, Y.

    7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. eLife, 10, e64872.

  • Jin Kai,et al

    BZW2 promotes malignant progression in lung adenocarcinoma through enhancing the ubiquitination and degradation of GSK3β

  • Berberine promotes peri‐implant osteogenesis in diabetic rats by ROS‐mediated IRS‐1 pathway

  • Antileukemic effect of caffeic acid 3, 4-dihydroxyphenetyl ester. Evidences for its mechanisms of action

  • CDC42‑mediated Wnt signaling facilitates odontogenic differentiation of DPCs during tooth root elongation

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