Phospho-GATA3 (S308) Recombinant Rabbit Monoclonal Antibody [ST44-09] (ET1609-17)
Catalog# ET1609-17
Rabbit Monoclonal to Phospho-GATA3 (S308)
Tested for
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WB
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IHC-P
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-GATA3 (S308) on different lysates with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate, treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-17) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate, treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-17) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.