Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SC58-01] (HA750199)
Catalog# HA750199
Rabbit Monoclonal to Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
Tested for
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) on different lysates with Rabbit anti-Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) antibody (HA750199) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lane 3: PC-12 cell lysate
Lane 4: PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41/43 kDa
Observed band size: 41/43 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750199) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) on different lysates with Rabbit anti-Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) antibody (HA750199) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lane 3: PC-12 cell lysate
Lane 4: PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41/43 kDa
Observed band size: 41/43 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750199) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.