Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)
Catalog# ET1610-13
Rabbit Monoclonal to Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lane 3: PC-12 cell lysate
Lane 4: PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41/43 kDa
Observed band size: 41/43 kDa
Exposure time: 1 minute 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-13) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)
Immunogen
Synthetic peptide within human aa 166-215 / 379.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, SK-Br-3 whole cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, HeLa treated with 200nM PMA for 30 minutes cell lysate, human thyroid carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC58-01
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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IF-Tissue
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1:200
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 41/43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
SYNONYMS
ERK 1 antibody;ERK 2 antibody;ERK-1 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;HS44KDAP antibody;HUMKER1A antibody;Insulin-stimulated MAP2 kinase antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase 3 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Microtubule-associated protein 2 kinase antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;Mitogen-activated protein kinase 3 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p41mapk antibody;p42-MAPK antibody;P42MAPK antibody;p44-ERK1 antibody;p44-MAPK antibody;p44ERK1 antibody;p44MAPK antibody;PRKM 2 antibody;PRKM1 antibody;PRKM2 antibody;PRKM3 antibody;protein tyrosine kinase ERK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
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