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Phospho-Erk1+Erk2 (Erk1(pT202/pY204)+Erk2(pT185/pY187)) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)

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Catalog# ET1610-13

Rabbit Monoclonal to Phospho-Erk1+Erk2 (Erk1(pT202/pY204)+Erk2(pT185/pY187))

Tested for

  • WB

  • IP

  • IHC-P

React with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-Erk1+Erk2 (Erk1(pT202/pY204)+Erk2(pT185/pY187)) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)

Immunogen

Synthetic peptide within human aa 166-215 / 379.

Host

Rabbit

Modification

Phospho

Modification Site

Erk1(pT202/pY204)+Erk2(pT185/pY187)

Positive Control

A431 cell lysate, MCF-7 cell lysate, SK-Br-3 cell lysate, Jurkat cell lysates, human thyroid carcinoma.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC58-01

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • Use at an assay dependent concentration.

  • IHC-P

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

41/43 kDa

Isotype

IgG

TARGET

UNIPROT #

P27361

PROTEIN NAME

Phospho-Erk1+Erk2 (Erk1(pT202/pY204)+Erk2(pT185/pY187))

SYNONYMS

ERK 1 antibody;ERK 2 antibody;ERK-1 antibody;ERK-2 antibody;ERK1 antibody;erk1/2 antibody;ERK2 antibody;ERT1 antibody;ERT2 antibody;Extracellular signal regulated kinase 1 antibody;Extracellular signal-regulated kinase 1 antibody;Extracellular signal-regulated kinase 2 antibody;HS44KDAP antibody;HUMKER1A antibody;Insulin-stimulated MAP2 kinase antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase 3 antibody;MAP kinase isoform p42 antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 2 antibody;MAPK 3 antibody;Mapk1 antibody;MAPK2 antibody;MAPK3 antibody;Microtubule-associated protein 2 kinase antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;Mitogen-activated protein kinase 3 antibody;MK01_HUMAN antibody;p38 antibody;p40 antibody;p41 antibody;p41mapk antibody;p42-MAPK antibody;P42MAPK antibody;p44-ERK1 antibody;p44-MAPK antibody;p44ERK1 antibody;p44MAPK antibody;PRKM 2 antibody;PRKM1 antibody;PRKM2 antibody;PRKM3 antibody;protein tyrosine kinase ERK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

CITATIONS

  • Chao Shen

    Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling

  • Yin, Y., Wang, J., Xu, X., Zhou, B., Chen, S., Qin, T., & Peng, D.

    Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine

  • Xiawei Wei

    Napabucasin,a novel inhibitor of STAT3,inhibits growth and synergises with doxorubicin in diffuse large B-cell lymphoma

  • Shen, Junyi et al.

    Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway. | Aging [2019]

  • Zong, X., Xiao, X., Shen, B., Jiang, Q., Wang, H., Lu, Z., Wang, F., Jin, M., Min, J., Wang, F., & Wang, Y.

    The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. Nucleic acids research, 49(10), 5537–5552.

  • Fan, R., Chen, C., Hu, J., Mu, M., Chuan, D., Chen, Z., Guo, G., & Xu, J.

    Multifunctional gold nanorods in low-temperature photothermal interactions for combined tumor starvation and RNA interference therapy

  • Pang, Y., Zhao, L., Ji, X., Guo, K., & Yin, X.

    Analyses of Transcriptomics upon IL-1β-Stimulated Mouse Chondrocytes and the Protective Effect of Catalpol through the NOD2/NF-κB/MAPK Signaling Pathway

  • Yuan, Y., Wang, X., Li, J., Han, L., Du, H., Sun, Y., Yang, P., Zhou, Z., Gu, M., Lu, Y., & Shen, C.

    Single-Cell Sequencing Yields Insights in the Evolution of Foot-and-Mouth Disease Virus Persistent Infection

  • Wen, Di et al.

    Molecular hydrogen attenuates methamphetamine-induced behavioral sensitization and activation of ERK-ΔFosB signaling in the mouse nucleus accumbens. | Progress in Neuro-Psychopharmacology & Biological Psychiatry [2020]

  • He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.

    PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis

  • Wen, J., Bao, Z., Li, L., Liu, Y., Wei, B., Ye, X., Xu, H., Cui, L., Li, X., Shen, G., Fang, Y., Zeng, H., Shen, Z., Guo, E., Jin, H., & Wu, L.

    Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects

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