PRODUCT CODE: ET1610-13

Phospho- Erk1(pT202/pY204)+Erk2(pT185/pY187) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: MCF-7 cell lysate<br />
Lane 3: SK-Br-3 cell lysate
  • Western blot analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: MCF-7 cell lysate<br />
Lane 3: SK-Br-3 cell lysate
Western blot analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SK-Br-3 cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho- Erk1(pT202/pY204)+Erk2(pT185/pY187) Recombinant Rabbit Monoclonal Antibody [SC58-01] (ET1610-13)

Immunogen

Synthetic peptide within human aa 166-215 / 379.

Host

Rabbit

Modification

Phospho

Modification Site

Erk1(pT202/pY204)+Erk2(pT185/pY187)

Positive Control

A431 cell lysate, MCF-7 cell lysate, SK-Br-3 cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC58-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

42/44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Phospho- Erk1(pT202/pY204)+Erk2(pT185/pY187)

SYNONYMS

ERK 1 antibody; ERK 2 antibody; ERK-1 antibody; ERK-2 antibody; ERK1 antibody; erk1/2 antibody; ERK2 antibody; ERT1 antibody; ERT2 antibody; Extracellular signal regulated kinase 1 antibody; Extracellular signal-regulated kinase 1 antibody; Extracellular signal-regulated kinase 2 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin-stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 2 antibody; MAP kinase 3 antibody; MAP kinase isoform p42 antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 2 antibody; MAPK 3 antibody; Mapk1 antibody; MAPK2 antibody; MAPK3 antibody; Microtubule-associated protein 2 kinase antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 2 antibody; Mitogen-activated protein kinase 3 antibody; MK01_HUMAN antibody; p38 antibody; p40 antibody; p41 antibody; p41mapk antibody; p42-MAPK antibody; P42MAPK antibody; p44-ERK1 antibody; p44-MAPK antibody; p44ERK1 antibody; p44MAPK antibody; PRKM 2 antibody; PRKM1 antibody; PRKM2 antibody; PRKM3 antibody; protein tyrosine kinase ERK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

CITATIONS

  • Wen, Di et al.

    Molecular hydrogen attenuates methamphetamine-induced behavioral sensitization and activation of ERK-ΔFosB signaling in the mouse nucleus accumbens. | Progress in Neuro-Psychopharmacology & Biological Psychiatry [2020]

  • Napabucasin,a novel inhi......

    Napabucasin,a novel inhibitor of STAT3,inhibits growth and synergises with doxorubicin in diffuse large B-cell lymphoma

  • Exopolysaccharide from C......

    Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling

  • Shen, Junyi et al.

    Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway. | Aging [2019]