Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE58-32] (HA721676)
Catalog# HA721676
Rabbit Monoclonal to Phospho-eIF4B (S406)
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-eIF4B (S406) on different lysates with Rabbit anti-Phospho-eIF4B (S406) antibody (HA721676) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 treated λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 80 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721676) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 treated λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 80 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721676) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.