Phospho-EGFR (Y1045) Recombinant Rabbit Monoclonal Antibody [PSH03-28] (HA721970)
Catalog# HA721970
Rabbit Monoclonal to Phospho-EGFR (Y1045)
Tested for
-
WB
-
IF-Cell
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FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-EGFR (Y1045) on different lysates with Rabbit anti-Phospho-EGFR (Y1045) antibody (HA721970) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721970) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-EGFR (Y1045) on different lysates with Rabbit anti-Phospho-EGFR (Y1045) antibody (HA721970) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721970) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.