Phospho-Chk2 (T68) Recombinant Rabbit Monoclonal Antibody [JE43-12] (HA721633)
Catalog# HA721633
Rabbit Monoclonal to Phospho-Chk2 (T68)
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WB
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Human
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Mouse
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Rat
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody (HA721633) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 25μM Etoposide for 5 hours cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721633) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Chk2 (T68) Recombinant Rabbit Monoclonal Antibody [JE43-12] (HA721633)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding thr68 of human chk2.
Host
Rabbit
Modification
Phospho
Modification Site
T68
Positive Control
HeLa treated with 20μM Etoposide for 2 hours cell lysate, Jurkat treated with 25μM Etoposide for 5 hours cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate, C6 treated with 25μM Etoposide for 5 hours cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE43-12
RRID
APPLICATION DILUTION
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WB
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 61 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Chk2 (T68)
SYNONYMS
CDS 1 antibody;Cds1 antibody;Cds1 homolog antibody;Checkpoint kinase 2 antibody;Checkpoint like protein CHK2 antibody;CHEK 2 antibody;Chek2 antibody;Chk 2 antibody;CHK2 checkpoint homolog (S. pombe) antibody;CHK2 checkpoint homolog antibody;CHK2_HUMAN antibody;hCds1 antibody;HuCds 1 antibody;LFS 2 antibody;LFS2 antibody;PP1425 antibody;RAD 53 antibody;RAD53 antibody;Rad53 homolog antibody;Serine/threonine protein kinase Chk2 antibody;Serine/threonine-protein kinase Chk2 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 is involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. Mutations to the CHEK2 gene have been linked to a wide range of cancers. The CHEK2 gene encodes for checkpoint kinase 2 (CHK2), a protein that acts a tumor suppressor. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2.[6] Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs). Thus, CHK2's inhibition of the CDC25 phosphatases prevents entry of the cell into mitosis. Furthermore, the CHK2 protein interacts with several other proteins including p53 (p53). Stabilization of p53 by CHK2 leads to cell cycle arrest in phase G1. Furthermore, CHK2 is known to phosphorylate the cell-cycle transcription factor E2F1 and the promyelocytic leukemia protein (PML) involved in apoptosis (programmed cell death).
CITATIONS
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TPP1 inhibits DNA damage response and chemosensitivity in esophageal cancer
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WB
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Human
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Citation
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