Phospho-CDK1 (T161) Recombinant Rabbit Monoclonal Antibody [PSH03-42] (HA721987)
Catalog# HA721987
Rabbit Monoclonal to Phospho-CDK1 (T161)
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-CDK1 (T161) on different lysates with Rabbit anti-Phospho-CDK1 (T161) antibody (HA721987) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 7: PC-12 cell lysate
Lane 8: PC-12 treated with UV for 1 hour cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721987) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-CDK1 (T161) on different lysates with Rabbit anti-Phospho-CDK1 (T161) antibody (HA721987) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 7: PC-12 cell lysate
Lane 8: PC-12 treated with UV for 1 hour cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721987) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.