Phospho-Cdc6 (S54) Recombinant Rabbit Monoclonal Antibody [SD08-47] (ET1612-96)
Catalog# ET1612-96
Rabbit Monoclonal to Phospho-Cdc6 (S54)
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of Phospho-Cdc6 (S54) on different lysates with Rabbit anti-Phospho-Cdc6 (S54) antibody (ET1612-96) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 3mM Hydroxyurea for 20 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Cdc6 (S54) Recombinant Rabbit Monoclonal Antibody [SD08-47] (ET1612-96)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser54 of human cdc6 aa 41-90 / 560.
Host
Rabbit
Modification
Phospho
Modification Site
S54
Positive Control
HeLa whole cell lysate, HeLa treated with 3mM Hydroxyurea for 20 hours whole cell lysate, Jurkat cell lysates, mouse spleen tissue, rat spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD08-47
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 63 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Cdc6 (S54)
SYNONYMS
Cdc 18L antibody;Cdc 6 antibody;CDC18 (cell division cycle 18, S.pombe, homolog) like antibody;CDC18 (S.pombe) antibody;Cdc18 related protein antibody;CDC18(S.pombe) antibody;Cdc18-related protein antibody;Cdc18L antibody;cdc6 antibody;CDC6 cell division cycle 6 homolog antibody;CDC6 related protein antibody;CDC6-related protein antibody;CDC6_HUMAN antibody;Cdc6p antibody;CELL CYCLE CONTROLLER CDC6 antibody;Cell division control protein 6 antibody;Cell division control protein 6 homolog antibody;Cell division cycle 6 homolog (S. cerevisiae) antibody;Cell division cycle 6 homolog antibody;Cell division cycle 6, S. cerevisiae, homolog of antibody;HsCDC 6 antibody;HsCDC18 antibody;HsCDC6 antibody;p62 antibody;p62(cdc 6) antibody;p62(cdc6) antibody
SEQUENCE SIMILARITIES
Belongs to the CDC6/cdc18 family.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Cdc6, or cell division cycle 6, is a protein in eukaryotic cells. CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA. It is thought that the CDC6-Cdt1 complex uses ATP hydrolysis to thread DNA through the central hole of the MCM doughnut. Mutations in the binding motif of CDC6 strongly suggest that ATP binding and hydrolysis is essential for its function. The minimal requirement for DNA binding has been mapped within its 47-amino acid sequence. Furthermore, Cdc6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase, thus nuclear division is suppressed.
CITATIONS
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Xiaofeng Zhu
Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation
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WB
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Human
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Citation
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