PRODUCT CODE: ET1612-74

Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50] (ET1612-74)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Phospho-CDC37 (S13) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: Jurkat cell lysate
  • Western blot analysis of Phospho-CDC37 (S13) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: Jurkat cell lysate
Western blot analysis of Phospho-CDC37 (S13) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Jurkat cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50] (ET1612-74)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser13 of human cdc37.

Host

Rabbit

Modification

Phospho

Modification Site

S13

Positive Control

NIH/3T3 cell lysate, Jurkat cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-50

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Phospho-CDC37 (S13)

SYNONYMS

CDC 37 antibody; Cdc37 antibody; CDC37 cell division cycle 37 homolog antibody; CDC37 cell division cycle 37 S cerevisiae homolog antibody; CDC37 cell division cycle 37, S cerevisiae, homolog of antibody; Cdc37 homolog antibody; CDC37 protein antibody; CDC37_HUMAN antibody; CDC37A antibody; cell division cycle 37 antibody; Cell division cycle 37 homolog antibody; Hsp90 chaperone protein kinase targeting subunit antibody; Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody; Hsp90 chaperone protein kinase-targeting subunit antibody; Hsp90 co chaperone Cdc37 antibody; Hsp90 co-chaperone Cdc37 antibody; p50 antibody; p50Cdc37 antibody; S cerevisiae hypothetical protein CDC37 antibody

SEQUENCE SIMILARITIES

Belongs to the CDC37 family.

POST-TRANSLATIONAL MODIFICATION

Constitutively sumoylated by UBE2I.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.