Phospho-CaMKII (T286) Recombinant Rabbit Monoclonal Antibody [JE51-81] (HA721794)
Catalog# HA721794
Rabbit Monoclonal to Phospho-CaMKII (T286)
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WB
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Human
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Mouse
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Rat
Western blot analysis of Phospho-CaMKII (T286) on different lysates with Rabbit anti-Phospho-CaMKII (T286) antibody (HA721794) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 30 µg/Lane.
Predicted band size: 54~73 kDa
Observed band size: 50~60 kDa
Exposure time: 53 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721794) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-CaMKII (T286) Recombinant Rabbit Monoclonal Antibody [JE51-81] (HA721794)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding thr286 of human camkii.
Host
Rabbit
Modification
Phospho
Modification Site
T286
Positive Control
Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE51-81
RRID
APPLICATION DILUTION
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WB
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1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-CaMKII (T286)
SYNONYMS
Calcium/calmodulin dependent protein kinase II alpha antibody;Calcium/calmodulin dependent protein kinase II beta antibody;Calcium/calmodulin dependent protein kinase II delta antibody;Calcium/calmodulin dependent protein kinase II gamma antibody;Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody;CaM kinase II alpha antibody;CaM kinase II antibody;CaM kinase II beta antibody;CaM kinase II delta antibody;CaM kinase II gamma antibody;CaM kinase II subunit alpha antibody;CaMK-II subunit alpha antibody;CAMK2 antibody;Camk2a antibody;CAMK2B antibody;CAMK2D antibody;CAMK2G antibody;CAMKA antibody;KCC2A_HUMAN antibody
SUBCELLULAR LOCATION
Synapse, Postsynaptic density, Cell projection, dendritic spine, dendrite.
FUNCTION
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/ threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β,γandδ, which may or may not be coexpressed in the same tissue types. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.
CITATIONS
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Na Liu,et al
Baicalein Ameliorates Insulin Resistance of HFD/STZ Mice through Activating PI3K/AKT Signal Pathway of Liver and Skeletal Muscle in a GLP-1R-Dependent Manner
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WB
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mice
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Citation
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