Phospho-c-Jun (S63) Recombinant Rabbit Monoclonal Antibody [SY0297] (ET1608-4)
Catalog# ET1608-4
Rabbit Monoclonal to Phospho-c-Jun (S63)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lane 1: C6 cell lysate
Lane 2: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate
Lane 5: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes, then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-c-Jun (S63) Recombinant Rabbit Monoclonal Antibody [SY0297] (ET1608-4)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser63 of human c-jun aa 31-80 / 331.
Host
Rabbit
Modification
Phospho
Modification Site
S63
Positive Control
C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, PC-3M, MCF-7, A549, human breast carcinoma tissue, human tonsil tissue, human colon carcinoma tissue, human endometrial tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY0297
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-c-Jun (S63)
SYNONYMS
Activator protein 1 antibody;AP 1 antibody;AP1 antibody;cJun antibody;Enhancer Binding Protein AP1 antibody;Jun Activation Domain Binding Protein antibody;JUN antibody;Jun oncogene antibody;JUN protein antibody;Jun proto oncogene antibody;JUN_HUMAN antibody;JUNC antibody;Oncogene JUN antibody;p39 antibody;Proto oncogene c jun antibody;Proto oncogene cJun antibody;Proto-oncogene c-jun antibody;Transcription Factor AP 1 antibody;Transcription factor AP-1 antibody;Transcription Factor AP1 antibody;V jun avian sarcoma virus 17 oncogene homolog antibody;V jun sarcoma virus 17 oncogene homolog (avian) antibody;V jun sarcoma virus 17 oncogene homolog antibody;V-jun avian sarcoma virus 17 oncogene homolog antibody;vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
SEQUENCE SIMILARITIES
Belongs to the bZIP family. Jun subfamily.
TISSUE SPECIFICITY
Expressed in the developing and adult prostate and prostate cancer cells.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.; Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.; Acetylated at Lys-271 by EP300.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
c-Jun is a protein that in humans is encoded by the JUN gene. c-Jun, in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the JUN gene. c-jun was the first oncogenic transcription factor discovered. The proto-oncogene c-Jun is the cellular homolog of the viral oncoprotein v-jun (P05411). The viral homolog v-jun was discovered in avian sarcoma virus 17 and was named for ju-nana, the Japanese word for 17. The human JUN encodes a protein that is highly similar to the viral protein, which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
CITATIONS
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Wang, B., Xu, J., Ren, Q., Cheng, L., Guo, F., Liang, Y., Yang, L., Tan, Z., Fu, P., & Ma, L.
Fatty acid-binding protein 4 is a therapeutic target for septic acute kidney injury by regulating inflammatory response and cell apoptosis
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IHC
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Mouse
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Citation
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Geng Xiaoyu,et al
Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai
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WB
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Mouse
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Citation
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Liu, Q., Xiang, P., Chen, M., Luo, Y., Zhao, Y., Zhu, J., Jing, W., & Yu, H.
Nano-Sized Hydroxyapatite Induces Apoptosis and Osteogenic Differentiation of Vascular Smooth Muscle Cells via JNK/c-JUN Pathway. International journal of nanomedicine, 16, 3633–3648.
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WB
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Human
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Citation
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Jian-Li Jiang,Hong-Yong Cu,Zhi-Nan Chen
Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis. Cellular oncology (Dordrecht), 42(4), 537–554.
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WB
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CO-IP
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IHC
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IF
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Human hepatocellular carcinoma cell
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Citation
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